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为提高石灰性土壤磷素利用率,采用平板分离法从山西石灰性土壤作物根际分离筛选出一株溶磷能力强的真菌Z60,结合该菌菌落形态特征和ITS rDNA序列分析,确定该溶磷菌株的系统发育地位,并且对Z60溶磷特性以及产酸特性进行了深入研究.鉴定菌株Z60为黑曲霉(Aspergillus niger);Z60对磷酸三钙、磷酸铝、磷酸铁和磷矿粉最高溶磷量分别为714.4、624.1、305.2和108.3 mg L-1;不同碳氮源条件下,分别以蔗糖和KNO3为碳源和氮源时对磷酸三钙溶解磷量最高,对碳源的利用顺序依次为蔗糖>葡萄糖>甘露醇>乳糖>淀粉,对氮源的利用顺序依次为KNO3>NH4NO3>NH4Cl>(NH4)2SO4>NaNO3.HPLC分析表明:Z60在各种氮源条件下都产生草酸,以铵态氮为氮源都产生甲酸,以NH4NO3为氮源同时产生乳酸,以NH4Cl为氮源还产生酒石酸,以KNO3为氮源还可以产生琥珀酸.因此,Z60对磷酸三钙等难溶态磷都有较强的溶解能力,有望开发为石灰性土壤微生物磷肥的优质菌株.
In order to improve the utilization rate of phosphorus in calcareous soil, a strain Z60 was isolated from the rhizosphere of calcareous soil in Shanxi Province by plate separation method. Based on its morphological characteristics and ITS rDNA sequence analysis, Phosphorus strains of the phylogenetic position, and Z60 phosphate solubilizing characteristics and acid-producing properties of an in-depth study identified strain Z60 aspergillus niger (Aspergillus niger); Z60 of tricalcium phosphate, aluminum phosphate, iron phosphate and the highest phosphate Under the conditions of different carbon and nitrogen sources, sucrose and KNO3 as carbon and nitrogen sources, respectively, had the highest amounts of dissolved phosphorus for tricalcium phosphate and the order of utilization of carbon sources Followed by sucrose> glucose> mannitol> lactose> starch. The order of utilization of nitrogen sources was KNO3> NH4NO3> NH4Cl> (NH4) 2SO4> NaNO3.HPLC analysis showed that Z60 produced oxalic acid under various nitrogen sources, When ammonium nitrogen is used as nitrogen source, formic acid is produced, while NH4NO3 is used as nitrogen source to produce lactic acid at the same time, NH4Cl is also used as nitrogen source to produce tartaric acid, and KNO3 is used as nitrogen source to produce succinic acid. Phosphorus has a strong ability to dissolve, is expected to open High-quality strains of calcareous soil microbial phosphate.