免疫组化染色技术已广泛应用于疾病的研究和诊断上，在免疫组化普及的今天，如何提高染色质量，建立标准化染色是非常重要的。本文对易被忽视的环节，如抗体分装、脱蜡、消化、阳性对照及自备试剂等进行了改进，现报告如下。l抗体分装将抗体从大包装内取出20～30UI于尖底离心管 Immunohistochemical staining techniques have been widely used in the research and diagnosis of diseases. Nowadays, immunohistochemistry is very important in improving the quality of dyeing and establishing the standard dyeing. This article easily overlooked aspects, such as antibody dispensing, dewaxing, digestion, positive control and self-contained reagents were improved, are as follows. l antibody aliquot the antibody from the large package removed 20 ~ 30UI in the tip of the centrifuge tube