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本文应用T细胞受体γ基因的V_γ和J_γ引物对一组急性淋巴细胞白血病(ALL)标本进行了DNA多聚酶链反应(PCR)的研究,应用不对称PCR以及DNA直接测序方法检测了18个中国人ALL细胞的TCR_γ基因V-J结合部区域(N顺序),在中国人ALL中首次证明了TCR_γ基因的N顺序确实是克隆特异的。进一步,依据已经测序的N顺序,我们合成了几个白血病克隆特异的寡核苷酸作为探针,对4例ALL进行了微小残余病变(MRD)的研究,显示该方法的灵敏度为0.1—0.01％。
In this study, DNA polymerase chain reaction (PCR) was performed on a group of acute lymphoblastic leukemia (ALL) specimens using V_γ and J_γ primers of the T cell receptor γ gene, and 18 Chinese were detected using asymmetric PCR and DNA direct sequencing methods. The TCR_γ gene VJ junction region (N sequence) of human ALL cells was the first to prove in Chinese ALL that the N sequence of the TCR_γ gene is indeed clone-specific. Further, based on the sequence of N sequences that have been sequenced, we synthesized several leukemia clone-specific oligonucleotides as probes and performed microscopic residual disease (MRD) studies on four cases of ALL. The sensitivity of the method was 0.1-0.01. %.