论文部分内容阅读
目的分析吉林省2000~2012年从急性弛缓性麻痹(Acute Flaccid Paralysis,AFP)病例粪便标本中分离的脊髓灰质炎(脊灰)病毒(Poliovirus,PV)的血清分型以及型内鉴别结果。方法原始粪便标本来自吉林省2000~2012年﹤15岁儿童AFP病例,使用转人PV受体的小鼠肺细胞系和人横纹肌肉瘤细胞进行病毒分离,阳性标本进行中和试验鉴定血清型,中和试验鉴定为阳性的PV株送中国疾病预防控制中心病毒病预防控制所国家脊灰实验室进行型内鉴别,型内鉴别方法采用聚合酶链反应-限制性酶切片段长度多态性分析和酶联免疫吸附试验或VP_1编码区核苷酸序列分析法。结果吉林省2000~2012年共采集804例AFP病例粪便标本,通过细胞培养的方法共有25例分离到PV,均为疫苗相关(Vaccine-associated)PV(VAPV),其中2008年1例AFP病例分离到Ⅱ型和Ⅲ型VAPV,其中的Ⅲ型PV为VP1编码区Ⅲ型和Ⅱ型疫苗重组PV。结论吉林省2000~2012年未发现脊灰野病毒或疫苗衍生脊灰病毒引起的AFP病例,为吉林省维持无脊灰提供了实验室依据。
Objective To analyze the serotyping and in-vivo differential diagnosis of poliovirus (PV) isolated from stool samples of Acute Flaccid Paralysis (AFP) from 2000 to 2012 in Jilin Province. Methods The original stool samples were from AFP cases of children aged <15 years from 2000 to 2012 in Jilin province. The virus was isolated from mouse lung cell lines transfected with PV receptor and human rhabdomyosarcoma cells. Positive samples were tested for neutralization by serogroups And tested positive for PV strains sent to the Chinese Center for Disease Control and Prevention virus prevention and control of the National Polio Laboratory identification of type, type identification method using polymerase chain reaction - restriction fragment length polymorphism and Enzyme-linked immunosorbent assay or nucleotide sequence analysis of VP_1 coding region. Results A total of 804 stool samples of AFP cases were collected from 2000 to 2012 in Jilin Province. A total of 25 cases of PV were isolated by cell culture. All of them were Vaccine-associated PV (VAPV), of which 1 case of AFP was isolated in 2008 To type II and type III VAPV, in which type III PV is VP1 coding region type III and type II vaccine recombinant PV. Conclusions No cases of AFP caused by poliovirus or vaccine-derived poliovirus were found in Jilin Province from 2000 to 2012, which provided a laboratory basis for maintaining polio-free in Jilin Province.