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在对大豆根系盐胁迫抑制差减杂交文库EST序列分析的基础上,利用RT-PCR技术克隆得到了一个大豆Us-pA基因,命名为GmUsp1,其编码一个包含164个氨基酸残基的多肽链。多序列比对和编码蛋白结构分析表明,GmUsp1属于泛应激蛋白(Universal Stress Protein)家族成员之一。其氨基酸序列与蚕豆Vf_enod18序列相似度最高,属于MJ-0577类中含有ATP结合位点的UspA亚族,与MJ-0577有相似的蛋白二级结构。分别采用250 mmol.L-1NaCl、100μmol.L-1ABA和30%PEG 6000进行胁迫处理,耐盐品种文丰7号和盐敏感品种Union的GmUsp1均被诱导表达,二者对胁迫诱导响应时间和表达量存在差异,说明GmUsp1可能参与大豆对非生物逆境胁迫的应答调控。
Based on the analysis of the EST sequence of the subtracted hybridization library with salt stress suppression in soybean root, a soybean Us-pA gene named GmUsp1 was cloned by RT-PCR and encoded a polypeptide chain with 164 amino acid residues. Multiple sequence alignments and structural analysis of the encoded proteins indicated that GmUsp1 belongs to one of the members of the Universal Stress Protein family. Its amino acid sequence has the highest similarity with Vf_enod18. It belongs to the UspA subfamily of ATP binding sites in MJ-0577 and has similar secondary structure to MJ-0577. The treatments of 250 mmol.L-1 NaCl, 100 μmol.L-1ABA and 30% PEG 6000 were used to induce the expression of GmUsp1, a salt tolerant variety Wenfeng 7 and the salt sensitive variety Union, respectively. The response time and The results indicated that GmUsp1 may be involved in the regulation of response to abiotic stress in soybean.