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目的:探讨P物质(P物质(substance P,SP)、c-fos参与慢性前列腺炎/慢性盆腔疼痛综合征(CP/CPPS)疼痛的可能机制。方法:自身免疫法建立CP/CPPS大鼠模型;Von Frey丝检测机械刺激缩足阈(PWT);免疫组化检测前列腺与脊髓L5~S2节段背角SP、c-fos的表达变化和相关性。结果:造模后大鼠PWT下降,与对照组相比差异有统计学意义[(48.83±1.55)g vs(67.98±5.44)g,(P<0.05)]。前列腺呈现显著炎症反应,在病变范围、间质淋巴细胞浸润程度方面都较对照组反应加重,差异有统计学意义(P<0.05)。与对照组相比,模型组前列腺与脊髓L5~S2背角中SP、c-fos阳性表达均上调,差异有统计学意义(P<0.05)。前列腺与脊髓中SP蛋白表达无相关性(r=0.099,P=0.338);c-fos蛋白表达亦无相关性(r=0.027,P=0.454)。结论:SP、c-fos可能通过脊髓L5~S2节段的表达上调参与了疼痛的形成和维持。CP/CPPS疼痛的发生过程中脊髓L5~S2节段背角的病理变化可能起着重要的作用。
Objective: To explore the possible mechanism of substance P (SP) and c-fos participating in chronic prostatitis / chronic pelvic pain syndrome (CP / CPPS) pain.Methods: CP / CPPS rat model was established by autoimmunity The PWT of Von Frey was detected by immunohistochemistry and the expressions of SP and c-fos in the segments of L5 ~ S2 segments of prostate and spinal cord were detected by immunohistochemistry.Results: (48.83 ± 1.55) g vs (67.98 ± 5.44) g, (P <0.05)]. The prostate showed a significant inflammatory reaction, both in the extent of the lesion and in the degree of interstitial lymphocyte infiltration (P <0.05) .Compared with the control group, the positive expression of SP and c-fos in the L5 ~ S2 dorsal horn of the model group were all up-regulated, the difference was statistically significant (P < (R = 0.027, P = 0.454) .Conclusion: There is no correlation between SP and c-fos protein expression in the prostate and spinal cord (r = 0.099, Fos may be involved in the formation and maintenance of pain through the upregulation of L5 ~ S2 segments in the spinal cord.Pathological changes of the spinal dorsal segment L5 ~ S2 in the process of CP / CPPS pain It may play an important role.