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延脑腹内侧头端(RVM)包括中缝核及其周围网状结构,具丰富的脑啡肽免疫活性能神经元及末梢,RVM 微量注射吗啡已证明可引起镇痛。近来发现,RVM 中有一群细胞(Off-cell),当烫尾引起甩尾反射(TF)时,放电完全被抑制,但电刺激RVM 时,则持续放电,而TF 消失。本文进一步证明,用吗啡也能解除TF 时off-cell 放电的抑制。大鼠(300～350 g)经戊巴比妥纳麻醉后,用单极钢针微电极插入RVM 用于记录及刺激。动物稍醒后,尾部45°热刺激使产生较恒定的TF。电极随后逐渐向RVM 腹侧推进,并同时用波宽400 MS 频率50Hz连续刺激,直至TF 被小于10 mA 电流刺激抑制为止,
The rostral ventrolateral medullary head (RVM), including the nucleus raphe nucleus and its surrounding network structure, is rich in enkephalin immunoreactive neurons and peripherals, RVM microinjection of morphine has been shown to cause analgesia. It has recently been discovered that there is a group of cells in the RVM that discharge is completely inhibited when tail flicking (TF) is induced by hot tailing, but the discharge continues while the RVM is electrically stimulated while TF disappears. This article further demonstrates that the inhibition of off-cell discharge can also be relieved with morphine. Rats (300-350 g) were anesthetized with pentobarbital, and monopolar needle microelectrodes were inserted into the RVM for recording and stimulation. After the animal wakes slightly, the tail 45 ° thermal stimulation produces a more constant TF. The electrode was then progressively advanced toward the ventral aspect of the RVM and simultaneously stimulated continuously at a frequency of 400 Hz with a wave length of 400 MS until the TF was inhibited by less than 10 mA of current stimulation,