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研究海蜇糖蛋白(JGP-III)对BALB/c小鼠脾脏淋巴细胞细胞因子mRNA表达的影响,探讨JGP-Ⅲ对小鼠的免疫调节机理。选用54只BALB/c小鼠(18~22g,SPF级),随机分为6组:模型对照组、正常对照组、阳性对照组(左旋咪唑25mg/(kg·d),以体质量计)、低剂量组(JGP-Ⅲ10mg/(kg·d))、中剂量组(JGP-Ⅲ50mg/(kg·d))和高剂量组(JGP-Ⅲ100mg/(kg·d)),每组9只。饲养21d后,无菌取脾脏,分离淋巴细胞,采用荧光定量RT-PCR法分析其细胞因子IL-2、IL-12、IFN-γ、IL-4、IL-6和IL-10 mRNA的相对表达量。结果显示:通过3个剂量组和3个对照组中细胞因子mRNA表达水平的对比,得出海蜇糖蛋白JGP-III能够促进细胞因子IL-2、IL-12、IFN-γ的mRNA表达水平,而抑制IL-4、IL-6和IL-10 mRNA的表达水平,且具有剂量依赖关系。进而推测JGP-Ⅲ可能是通过调节淋巴细胞Th1/Th2细胞因子mRNA的表达水平,来提高机体免疫能力。
To investigate the effect of JGP-III on the cytokine mRNA expression in splenic lymphocytes of BALB / c mice and to explore the immunomodulatory mechanism of JGP-III in mice. 54 BALB / c mice (18-22g, SPF grade) were randomly divided into 6 groups: model control group, normal control group and positive control group (levamisole 25mg / (kg · d), body weight) , Low dose group (JGP-Ⅲ10mg / (kg · d)), middle dose group (JGP-Ⅲ50mg / (kg · d)) and high dose group (JGP-Ⅲ100mg / (kg · d) . After feeding for 21 days, the spleens were removed aseptically and the lymphocytes were isolated. The relative expression of cytokines IL-2, IL-12, IFN-γ, IL-4, IL-6 and IL- The amount of expression. The results showed that JGP-III of jellyfish jelly could promote the mRNA expression of IL-2, IL-12 and IFN-γ by comparing the mRNA expression levels of cytokines in three dose groups and three control groups, But inhibited the expression of IL-4, IL-6 and IL-10 mRNA in a dose-dependent manner. Furthermore, it is speculated that JGP-Ⅲ may enhance the immunity by regulating the expression of Th1 / Th2 cytokines in lymphocytes.