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为探讨衰老过程中转录活性水平下降的机理，用同位素掺入法和脱氧核糖核酸酶Ⅰ（ＤｅｏｘｙｒｉｂｏｎｕｃｌｅａｓＩ，ＤＮａｓｅⅠ）作为检测基因活性的探针，对人胚肺成纤维细胞２ＢＳ在衰老过程中染色质转录活性的变化进行研究。衰老细胞核染色质体外转录活性较年轻细胞下降５８％，并对ＤＮａｓｅＩ的敏感性也较年轻组有明显降低。提示：衰老２ＢＳ核染色质结构的改变可能是转录活性改变的重要因素。
In order to explore the mechanism of the decline of transcriptional activity in the process of aging, isotope incorporation and Deoxyribonucleic acid Ⅰ (DNase Ⅰ) were used as probes to detect gene activity. Chromatin Changes in transcriptional activity were investigated. Senescent nuclear chromatin in vitro transcription activity decreased 58% compared with young cells, and DNaseI sensitivity than the younger group was significantly lower. Tip: Changes in the chromatin structure of aging 2BS nucleus may be an important factor in the transcriptional change.