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目的研究苏丹Ⅲ对小鼠骨髓细胞的遗传毒性作用,探索苏丹Ⅲ对小鼠骨髓细胞增殖和染色体稳定性的影响。方法制备含有不同剂量苏丹Ⅲ的干粮喂饲小白鼠30d,取小鼠骨髓细胞制备染色体标本,观察骨髓细胞增殖、染色体结构畸变等指标。计算并统计分析细胞增殖指数、细胞畸变数和染色体畸变率。同时,观察小鼠体重及心、肝、肾器官重量的变化。结果染毒高、中和低剂量组的骨髓细胞增殖指数分别是16.13%,34.82%和37.68%,与对照组比较均有下降,差异有统计学意义(P<0.01);染色体畸变率在高、中和低剂量组分别是7.17%,7.00%和5.17%,与对照组比较,差异有统计学意义(P<0.05,P<0.01);细胞畸变率高、中和低剂量组分别是6.83%,6.67%和3.67%,与对照组比较,差异有统计学意义(P<0.05,P<0.01);而染毒各剂量组小鼠的心、肝、肾的脏体系数与正常组比较,差异无显著性。结论在本实验条件下,苏丹Ⅲ对小鼠骨髓的细胞增殖有明显抑制作用,并可导致小鼠骨髓细胞畸变率及染色体畸变率增高,但不影响染色体的稳定性。
Objective To study the genotoxic effect of Sudan Ⅲ on mouse bone marrow cells and explore the effect of Sudan Ⅲ on the proliferation and chromosome stability of mouse bone marrow cells. Methods Dry mice fed with different doses of Sudan Ⅲ were fed with mice for 30 days. The bone marrow cells of mice were used to prepare the chromosome samples. The proliferation and chromosome aberrations of bone marrow were observed. Calculated and statistical analysis of cell proliferation index, cell aberration and chromosome aberration rate. At the same time, observe the weight of mice and heart, liver and kidney organ weight changes. Results The proliferation index of bone marrow cells in high, medium and low dose groups were 16.13%, 34.82% and 37.68%, respectively, which were significantly lower than those in control group (P <0.01). The chromosome aberration rate was high , Medium and low dose groups were 7.17%, 7.00% and 5.17%, respectively, compared with the control group, the difference was statistically significant (P <0.05, P <0.01); cells with high, medium and low dose groups were 6.83 %, 6.67% and 3.67%, respectively, compared with the control group, the difference was statistically significant (P <0.05, P <0.01); while the body weight of heart, liver and kidney in each dose group was significantly lower than that in the normal group , No significant difference. Conclusion Under the experimental conditions, Sudan Ⅲ significantly inhibits the proliferation of mouse bone marrow cells, and can lead to increased bone marrow cell aberrations and chromosome aberrations in mice, but does not affect the stability of chromosomes.