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根据对流免疫电泳的原理,设计了一个经聚丙烯酰胺凝胶电泳(PAGE)分离后未变性蛋白免疫学检测方法——对流免疫印染(CIB)。CIB法,即将蛋白自聚丙烯酰胺凝胶转移至含抗体的琼脂糖胶内,形成的抗原抗体复合物可进一步用染色或放射自显影鉴别。作者先制备一个1mm厚的琼脂糖凝胶(在相距1mm的10.5×10cm~2玻片之间倒胶),再制备一层1mm厚含抗体的凝胶(可制作含不同抗体的凝胶板并在一起,但要防止互相污染)。PAGE后,将PAG、不含抗体的琼脂糖胶、含抗体的琼脂糖胶,以及硝酸
According to the principle of convective immuno-electrophoresis, a non-denatured protein immunological detection method - convective immunoblotting (CIB) was designed after polyacrylamide gel electrophoresis (PAGE). CIB method, that is, the protein is transferred from the polyacrylamide gel to the antibody-containing agarose gel, and the antigen-antibody complex formed can be further identified by staining or autoradiography. The authors first prepared a 1mm thick agarose gel (10.5x10cm ~ 2mm away from the slide between the glass slide), and then prepare a layer of 1mm thick antibody-containing gel (can be made with different antibody gel plates And together, but to prevent mutual pollution). PAGE, PAG, antibody-free agarose gel, antibody-containing agarose gel, and nitric acid