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[目的]探讨常用防腐剂苯甲酸、苯甲酸钠、山梨酸钾、对羟基苯甲酸乙酯、氟化钠等对大鼠肝脏脂质过氧化(LPO)作用的影响。[方法]模拟苯甲酸、苯甲酸钠、山梨酸钾、对羟基苯甲酸乙酯、氟化钠等防腐剂在食物中的含量,配制一系列浓度,运用体外TBA比色法检测LPO的含量,了解常用防腐剂对大鼠肝脏的损害程度。[结果]在实验条件下苯甲酸在0.1～10.0mmol/L、苯甲酸钠在0.1～10.0mmol/L、山梨酸钾在1.0～20mmol/L、对羟基苯甲酸乙酯在1.0～10mmol/L、氟化钠在0.3～1.0mmol/L浓度范围内对大鼠肝脏LPO有显著的诱导作用。[结论]苯甲酸、苯甲酸钠、山梨酸钾、对羟基苯甲酸乙酯、氟化钠对大鼠肝脏LPO具有不同程度的诱导作用,会加大对肝脏细胞的损伤。
[Objective] To investigate the effect of commonly used preservatives such as benzoic acid, sodium benzoate, potassium sorbate, ethyl p-hydroxybenzoate and sodium fluoride on rat liver lipid peroxidation (LPO). [Method] The contents of benzoic acid, sodium benzoate, potassium sorbate, ethyl p-hydroxybenzoate and sodium fluoride in food were simulated and a series of concentrations were prepared. The content of LPO was detected by TBA colorimetry in vitro Common preservatives on rat liver damage. [Result] Under the conditions of 0.1-10.0 mmol / L benzoic acid, 0.1-10.0 mmol / L sodium benzoate, 1.0-20 mmol / L potassium sorbate, 1.0-10 mmol / L ethyl p - hydroxybenzoate, Sodium fluoride in the 0.3 ~ 1.0mmol / L concentration range of rat liver LPO significant induction. [Conclusion] Benzoic acid, sodium benzoate, potassium sorbate, ethyl p-hydroxybenzoate and sodium fluoride induce LPO in rat liver to some extent, which will increase the damage to liver cells.