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目的 研究 p16抑癌基因与卵巢上皮性浆液性肿瘤发生与发展的关系。 方法 应用原位杂交和免疫组化法 ,对 47份卵巢上皮性浆液性肿瘤组织和 8份正常卵巢组织 p16mRNA与 p16蛋白进行检测。 结果 p16mRNA与 p16蛋白阳性表达皆主要位于胞浆内 ,呈红色颗粒状 ,阳性表达细胞多呈灶状分布。p16mRNA与 p16蛋白在卵巢浆液性癌中检出率 (4 8 5 %、42 4% )皆低于良性浆液性瘤 (均 90 0 % )及正常卵巢组织 (均 10 0 0 % ) ,均P<0 0 5。p16mRNA、p16蛋白低表达与卵巢浆液性癌的恶性程度高、肿瘤分化差、癌瘤播散及淋巴结转移有关。一致性检验显示 p16基因的原位杂交和免疫组化检测结果关系密切 (P <0 0 1)。结论 p16作为一种新型抑癌基因 ,其mRNA或蛋白低表达与卵巢浆液性癌发生、发展密切相关。
Objective To study the relationship between p16 tumor suppressor gene and the occurrence and development of ovarian epithelial serous tumors. Methods In situ hybridization and immunohistochemistry were used to detect p16 mRNA and p16 protein in 47 ovarian epithelial serous tumors and 8 normal ovarian tissues. Results The positive expression of p16 mRNA and p16 protein were mainly located in the cytoplasm, with red granule. Most of the positive cells showed focal distribution. The positive rates of p16 mRNA and p16 protein in ovarian serous carcinoma (48.5%, 42.4%) were lower than those in benign serous tumors (90%) and normal ovarian tissues (100%), respectively <0 0 5. Low expression of p16 mRNA and p16 protein is associated with the high degree of malignancy of ovarian serous carcinoma, poorly differentiated tumor, cancer spread and lymph node metastasis. Consensus tests showed that the in situ hybridization of p16 gene and immunohistochemistry results were closely related (P <0.01). Conclusion As a novel tumor suppressor gene, p16 is closely related to the occurrence and development of ovarian serous carcinoma.