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本文特通过序列分析证实的IL-8基因亚克隆到表达载体p~(BV220)中,构建成了重组表达质粒;通过温度诱导表达,SDS-PAGE电泳后获得的新生蛋白带与预计表达的IL-8分子相吻合,表达产物主要聚集在胞屑间隙中,白细胞趋化实验初步测定了表达产物的生物学活性.
In this paper, the IL-8 gene confirmed by sequence analysis was subcloned into the expression vector p ~ (BV220) and constructed into a recombinant expression plasmid. The temperature induced expression of the nascent protein band obtained after SDS-PAGE electrophoresis was consistent with the expected expression of IL -8 molecules coincide, the expression product mainly gathered in the chip clearance, leukocyte chemotaxis assay preliminary determination of the biological activity of the expression product.