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本研究的目的是用高磷饲料(普通饲料加NaH_2PO_4·2H_2O,使磷含量为1％)诱发大鼠肾内钙化时,对其钙化灶的形成与分布作组织学动态观察。实验分三组,第Ⅰ组:雄大鼠57只,分为9组,每组6至7只,Ⅰ-1组于实验当日喂高磷饲料前处死;Ⅰ-2至Ⅰ-9组依次分别于实验后第3、5、7、9、16、24、32、50天处死,取左肾的一半(余肾做生化分析),及心、肝、脾、肺、胰、小肠、大肠,石腊包
The purpose of this study was to use a high-phosphorus diet (normal diet plus NaH 2 PO 4 ·2H 2 O, phosphorus content of 1%) to induce intrarenal calcification in rats, the formation and distribution of its calcification for histological dynamic observation. The experiment was divided into three groups. The first group consisted of 57 male rats divided into 9 groups with 6 to 7 rats in each group. The group I-1 was sacrificed before feeding high phosphorus diet on the day of the experiment; the I-2 to I-9 groups were divided into At the 3rd, 5th, 7th, 9th, 16th, 24th, 32nd, and 50th days after the experiment, they were sacrificed and half of the left kidney was taken (biochemical analysis of the remaining kidneys), as well as heart, liver, spleen, lung, pancreas, small intestine, and large intestine. Paraffin