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目的:寻找人肝细胞癌(HCC)特异抗原.方法:应用“重组子表达抗原克隆的血清学鉴定技术(SEREX)”,用病人自身血清从广西肝细胞癌cDNA文库中筛选出编码HCC肿瘤抗原的基因克隆,用肝癌患者及其他人血清检验HCC肿瘤抗原对肝癌的特异性,测定抗原DNA序列并与基因库核对寻找其同源结构.结果:其中一种HCC肿瘤抗原与衰老标记蛋白-30(SMP-30)是同源结构,相应抗体主要只见于HCC病人,在20例其它5种恶性肿瘤和4例肝硬化病人未查到相应抗体,20例慢性肝
OBJECTIVE: To search for human hepatocellular carcinoma (HCC)-specific antigens. METHODS: Using the serological identification technique for the expression of recombinant clones for expression of antigens (SEREX), we used the patient’s own serum to screen HCC tumor antigens from Guangxi hepatocellular carcinoma cDNA libraries. The gene clones were used to test the specificity of HCC tumor antigens for liver cancer using hepatocellular carcinoma patients and others’ sera. The antigen DNA sequences were determined and checked against the gene bank for homologous structures. Results: One of the HCC tumor antigens and senescence marker protein-30 (SMP-30) is a homologous structure, and the corresponding antibodies were mainly found in HCC patients. No antibodies were found in 20 cases of other 5 malignancies and 4 cases of cirrhosis and 20 cases of chronic liver.