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目的:改进大白兔VX2肝癌模型制作方法并研究肝癌组织缺血再灌注后的损伤作用。方法:用特殊技术方法制备高浓度细胞悬液,分别以27号注射针头向动物肝脏左中叶注射0.3 mL,制成动物肝癌模型。待肿瘤细胞种植成功至实验标准后,阻断肝左中叶之供血和门静脉60分钟,恢复血流至各个实验时间点。检测癌组织中CAT和SOD浓度的改变和细胞凋亡情况。结果:全部动物肝脏VX2细胞悬液注射后均种植成功但每组动物中有2-3只出现肿瘤细胞外溢和种植。种植成功之肿瘤组织缺血再灌注后CAT浓度明显降低且至1天达最低水平。SOD浓度在正常肝组织和肝癌组织中均明显降低而肝癌组织在再灌注后7天气浓度仍然保持较低水平。在正常肝组织和肝癌组织中肿瘤细胞凋亡均有增加,但在后者更为显著。结论:以特殊外科方法直接向肝组织内注射VX2细胞悬液能够成功制作大白兔肝癌模型。缺血再灌注可以导致组织的细胞凋亡,但对肝癌组织的影响更大。
OBJECTIVE: To improve the method of making VX2 liver cancer model in rabbits and to study the injury effect of HCC tissue after ischemia-reperfusion. Methods: High-concentration cell suspensions were prepared by special techniques and injected with 0.3 mL into the left middle lobe of the animal by using a 27-gauge needle to make an animal liver cancer model. After the tumor cells were successfully planted to the experimental standard, the blood supply to the left middle lobe and the portal vein were blocked for 60 minutes to restore the blood flow to each experimental time point. Detection of cancer tissue CAT and SOD concentration changes and apoptosis. RESULTS: All animal liver VX2 cell suspensions were seeded successfully but 2-3 of the animals in each group developed tumor cell spillage and implantation. The successful implantation of tumor tissue after ischemia-reperfusion CAT concentration was significantly reduced and reached the lowest level of 1 day. The SOD concentration in normal liver tissue and liver cancer tissue were significantly lower than that in hepatic cancer tissue after 7-day reperfusion remained at a low level. Tumor cell apoptosis increased in normal liver and liver cancer tissues, but was more pronounced in the latter. CONCLUSION: The injection of VX2 cell suspension directly into the liver tissue by special surgical methods can successfully produce a model of liver cancer in rabbits. Ischemia-reperfusion can lead to apoptosis of the tissue, but has a greater impact on liver cancer tissue.