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It has been well-documented that the distribution of ammonia-oxidizing bacteria (AOB) and archaea (AOA) in soils can be affected by heavy metal contamination,whereas information about the impact of heavy metal on these ammonia-oxidizing microorganisms in freshwater sediment is still lacking.The present study explored the change of sediment ammonia-oxidizing microorganisms in a freshwater reservoir after being accidentally contaminated by industrial discharge containing high levels of metals.Bacterial amoA gene was found to be below the quantitative PCR detection and was not successfully amplified by conventional PCR.The number of archaeal oA gene in reservoir sediments were 9.62 × 102-1.35 × 107 copies per gram dry sediment.AOA abundance continuously decreased,and AOA richness,diversity and community structure also considerably varied with time.Therefore,heavy metal pollution could have a profound impact on freshwater sediment AOA community.This work could expand our knowledge of the effect of heavy metal contamination on nitrification in natural ecosystems.