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目的:观察12种广东地产清热解毒药抑制EB病毒(EBV)壳抗原在体外细胞中表达的作用及对B95-8细胞的细胞毒作用。方法:应用间接免疫酶法研究12种中药对B95-8细胞壳抗原表达的抑制作用;采用台盼蓝拒染试验,观察中药对B95-8细胞在激发培养环境下的细胞毒作用。结果:12种广东地产清热解毒药在无细胞毒浓度下对激发条件下培养的B95-8细胞具有明显的抑制其VCA表达的作用,大部分药物呈现出药量依赖性;相应药物增加药量1 000倍,12种中药均对B95-8细胞有明显的细胞毒性作用,呈显著的药量依赖性。结论:12种广东地产清热解毒药抑制EBV壳抗原表达效果较好,高浓度时均对B95-8细胞具有细胞毒作用。
Objective: To observe the effect of detoxification drugs of Guangdong real estate on inhibiting the expression of Epstein - Barr virus (EBV) shell antigen in vitro and the cytotoxic effect on B95-8 cells. Methods: Indirect immunoenzymatic method was used to study the inhibitory effect of 12 Chinese herbs on the expression of B95-8 cell shell antigen. The trypan blue exclusion assay was used to observe the cytotoxic effect of traditional Chinese medicine on B95-8 cells under stimulated culture. Results: Twelve Guangdong real estate heat-clearing and antidotal drugs had no effect on VCA expression in B95-8 cells cultured under the conditions of no cytotoxicity. Most of the drugs exhibited a dose-dependent manner. Corresponding drugs increased the dosage 1 000 times, 12 kinds of Chinese medicine have obvious cytotoxicity on B95-8 cells, showed a significant dose-dependent. CONCLUSION: Twelve Guangdong real estate antipyretic drugs can inhibit the expression of EBV shell antigen better, and all have cytotoxic effects on B95-8 cells at high concentration.