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目的:考察HPLC法同时检测心脑健片中儿茶素、没食子儿茶素和表没食子儿茶素的含量。方法:色谱条件为Benetnach~(TM)C_(18)(250 mm×4.6 mm,5μm)色谱柱;流动相:A:水∶乙腈∶磷酸=95.0∶4.5∶0.5,B:水∶乙腈∶磷酸=49.0∶0.5∶0.5,梯度洗脱;流速:1.0 m L/min;检测波长:270 nm;柱温:25℃;进样量:20μL。结果:4个批号的心脑健片中儿茶素、没食子儿茶素和表没食子儿茶素的含量分别为24.1~27.7 mg/片、15.9~18.5 mg/片和12.2~17.3 mg/片。结论:儿茶素、没食子儿茶素和表没食子儿茶素的HPLC同时检测为心脑健片的质量控制提供了参考。
OBJECTIVE: To investigate the simultaneous determination of catechin, epigallocatechin and epigallocatechin in Xinnaojian Tablets by HPLC. METHODS: The chromatographic conditions were Benetnach TM C 18 column (250 mm × 4.6 mm, 5 μm); mobile phase: A: water: acetonitrile: phosphoric acid = 95.0: 4.5: 0.5, B: water: acetonitrile: phosphoric acid = 49.0:0.5:0.5, gradient elution; flow rate: 1.0 m L / min; detection wavelength: 270 nm; column temperature: 25 ° C; injection volume: 20 μL. Results: The content of catechin, epigallocatechin and epigallocatechin in four batches of Xinnaojian Tablets were 24.1-27.7 mg / tablet, 15.9-18.5 mg / tablet and 12.2-17.3 mg / tablet, respectively. Conclusion: The simultaneous HPLC detection of catechin, epigallocatechin and epigallocatechin can provide reference for the quality control of Xinnao Jianjian Tablets.