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目的探讨血管内皮生长因子VEGF及其受体KDR在胃癌新生血管形成中的作用。方法应用免疫组织化学技术,检测60例胃癌组织VEGF及KDR蛋白表达和以CD105标记的微血管密度,分析VEGF、KDR和MVD及其与肿瘤大小、肿瘤分化、浸润深度、淋巴结转移、组织学分级和预后关系。结果VEGF及KDR阳性者MVD值显著高于阴性者(t=3.85,t=3.76,P<0.01)。VEGF、KDR表达和MVD与胃癌浸润深度(χ2=8.31,P<0.01;χ2=5.05,P<0.05;t=2.24,P<0.05)、淋巴结转移(χ2=10.62,P<0.01;χ2=5.66,P<0.05;t=5.19,P<0.05)、组织学分级(χ2=11.05,P<0.01;χ2=8.21,P<0.01;t=2.11,P<0.05)密切相关。VEGF及KDR表达阳性或高MVD的胃癌患者5年生存率较低(χ2=5.18,P<0.05;χ2=8.94,P<0.01;t=3.51,P<0.01)。结论VEGF及KDR与胃癌新生血管生成密切相关,对胃癌的生长和浸润转移有促进作用,VEGF及KDR和以CD105标记的MVD可作为反映胃癌生物学行为的客观指标。
Objective To investigate the role of vascular endothelial growth factor VEGF and its receptor KDR in neovascularization of gastric cancer. Methods Immunohistochemical technique was used to detect the expression of VEGF and KDR protein in 60 cases of gastric carcinoma and the microvessel density marked by CD105. The expressions of VEGF, KDR and MVD in tumor tissues, tumor differentiation, depth of invasion, lymph node metastasis, histological grade and lymph node metastasis Prognosis Results The MVD of VEGF and KDR positive group was significantly higher than that of negative group (t = 3.85, t = 3.76, P <0.01). The expression of VEGF, KDR and the infiltration depth of MVD and gastric cancer (χ2 = 8.31, P0.01; χ2 = 5.05, P <0.05; t = 2.24, , P <0.05; t = 5.19, P <0.05), histological grade (χ2 = 11.05, P <0.01; χ2 = 8.21, P <0.01; t = 2.11, P <0.05). The 5-year survival rate of gastric cancer patients with positive VEGF or KDR expression or high MVD was lower (χ2 = 5.18, P <0.05; χ2 = 8.94, P <0.01; t = 3.51, P <0.01). Conclusions VEGF and KDR are closely related to angiogenesis of gastric cancer, which may promote the growth and infiltration and metastasis of gastric cancer. VEGF and KDR and MVD labeled with CD105 may serve as objective indicators reflecting the biological behavior of gastric cancer.