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测定海藻酸钠 多聚赖氨酸 海藻酸钠 (APA)微囊对免疫细胞和细胞因子的隔离效应。方法 :将NK细胞和IL 2、TNF α的活性测定方法用于微囊免疫隔离效果的评价。结果 :NK细胞对微囊化K5 6 2靶细胞的细胞毒实验表明 ,微囊可有效地保护囊内细胞不受NK细胞的杀伤作用 ;IL 2 (15 .4kD)和TNF α (5 1kD)可通过微囊膜进入囊内 ,并分别支持囊内IL 2依赖细胞的生存以及对微囊内的L92 9靶细胞发挥杀伤作用。结论 :APA微囊可有效地隔离细胞免疫排斥反应 ,但某些细胞因子可通过微囊膜 ,成为影响微囊化细胞在宿主体内生存的可能因素。
To determine the isolation effect of sodium alginate polylysine alginate (APA) microcapsules on immune cells and cytokines. Methods: The assay of NK cell, IL 2 and TNFα activity was used to evaluate the immune isolation effect of microcapsules. Results: Cytotoxicity test of NK cells on the microencapsulated K5 6 2 target cells showed that the microencapsulation could effectively protect the intracapsular cells from the cytotoxicity of NK cells. IL 2 (15.4kD) and TNFα (51kD) Can enter the capsule through the microcapsule membrane and support IL2-dependent cell survival within the capsule and killing of L92 & lt; 9 & gt; target cells within the microcapsule, respectively. Conclusion: APA microcapsules can effectively isolate the cellular immune rejection. However, some cytokines may pass through the microcapsule membrane and become a possible factor that affects the survival of microencapsulated cells in the host.