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目的 建立骨髓基质干细胞(marrowstromalcell,MSC)内耳移植技术,研究内耳细胞移植的可行性。方法 将豚鼠骨髓基质干细胞分离培养,用4 ,6 -联脒- 2 -苯基吲哚( 4 ,6 -diamidino - 2 -phenylindole,DAPI)荧光标记,采用显微注射技术在耳蜗底周鼓阶钻孔,通过钻孔处缓缓注入细胞,术后14天处死,行耳蜗石腊切片,荧光显微镜及HE染色观察移植细胞成活情况。结果 骨髓基质干细胞内耳移植成活,并贴壁生长。结论 成功地建立了骨髓基质干细胞内耳移植技术,为进一步研究MSC在内耳的分化和表达奠定了重要的基础
Objective To establish bone marrow mesenchymal stem cells (MSCs) transplantation in the inner ear and to study the feasibility of transplanting the inner ear cells. Methods Guinea pig bone marrow stromal stem cells were isolated and cultured, and labeled with 4, 6 - diamidino - 2 - phenylindole (DAPI) by microinjection. Drilled holes were slowly infused into the cells through the drill hole. After 14 days of operation, the cells were sacrificed and the paraffin sections of the paraffin sections were observed. The survival of the transplanted cells was observed by fluorescence microscope and HE staining. Results Bone marrow stromal stem cells were transplanted into the inner ear and grew adherently. Conclusion Successful establishment of bone marrow stromal stem cells transplantation in the inner ear provides an important foundation for further study on the differentiation and expression of MSC in the inner ear