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目的:用高效液相色谱法测定妇康片中阿魏酸的含量。方法:采用高效液相色谱法,色谱柱为C18柱(4.6mm×150mm,5μm),甲醇-1%冰醋酸(28:72)为流动相,流速为1.0mL.min-1,检测波长:323nm;进样量:10μL。结果:阿魏酸在0.05～0.25μg范围内与峰面积呈良好的线性关系,r=0.9999,平均回收率99.76%,RSD=1.08%(n=6)。结论:该方法精密度高,稳定性较好,结果准确可靠,可以用于妇康片的质量检验方法。
Objective: To determine the content of ferulic acid in Fukang Tablets by HPLC. Methods: The HPLC method was used. The column was C18 column (4.6mm×150mm, 5μm). The methanol-1% acetic acid (28:72) was used as the mobile phase. The flow rate was 1.0mL.min-1. The detection wavelength was: 323 nm; injection volume: 10 μL. Results: The ferulic acid showed a good linear relationship with the peak area in the range of 0.05-0.25 μg, r=0.9999, the average recovery rate was 99.76%, and RSD was 1.08% (n=6). Conclusion: This method has high precision, good stability, and accurate and reliable results. It can be used for quality inspection of Fukang tablets.