论文部分内容阅读
目的:探讨NRP-1单抗联合多西他赛节律化疗对胃癌裸鼠移植瘤的抗肿瘤疗效。方法:BALB/c裸鼠皮下接种胃癌BGC-823细胞制备移植瘤模型,将荷瘤裸鼠以数字随机表法随机分为对照组、NRP-1单抗组、节律化疗组(MCT)、联合组(NRP-1m Ab+MCT),每组6只。除对照组,其余各组于造模第8天开始分别给予相应治疗,给药2周,观察裸鼠一般状况,隔天测量裸鼠体重及肿瘤体积。裸鼠处死后称瘤质量,H-E染色观察瘤组织形态,免疫组化检测裸鼠瘤组织中NRP-1蛋白、VEGF、MVD表达。结果:联合组移植瘤的体积和质量显著低于其他各组[(0.394±0.128)vs(0.748±0.152)、(0.867±0.361)、(1.247±0.494)g;(0.613±0.223)vs(0.866±0.115)、(1.098±0.343)、(1.474±0.644)cm~3。均P<0.05],抑瘤率较其他治疗组差异有统计学意义(P<0.05)。对照组癌细胞生长良好,血管丰富,给药组癌组织出现不同程度的片状坏死,血管成分减少。免疫组化染色显示,对照组NRP-1表达明显高于治疗各组(P<0.05),联合组的NRP-1、VEGF、MVD表达均显著低于其余各组(P<0.05)。结论:NRP-1单抗联合多西他赛节律化疗可能通过下调NRP-1表达而显著抑制BGC-823胃癌移植瘤的生长及血管生成。
Objective: To investigate the anti-tumor effect of NRP-1 monoclonal antibody combined with docetaxel rhythm chemotherapy on transplanted gastric cancer in nude mice. METHODS: BALB / c nude mice were inoculated subcutaneously with BGC-823 gastric cancer cells to establish a transplanted tumor model. The nude mice bearing tumor were randomly divided into control group, NRP-1 monoclonal antibody group, MCT group, Group (NRP-1m Ab + MCT), 6 in each group. In addition to the control group, the remaining groups were given the appropriate treatment on the 8th day after the model was established. After 2 weeks of administration, the general condition of nude mice was observed. The body weight and tumor volume of the nude mice were measured every other day. The nude mice were sacrificed and the tumor mass was counted. The tumor morphology was observed by H-E staining. The expression of NRP-1 protein, VEGF and MVD in nude mice was detected by immunohistochemistry. Results: The volume and the quality of the transplanted tumor in the combined group were significantly lower than those in other groups [(0.394 ± 0.128) vs (0.748 ± 0.152), (0.867 ± 0.361) and (1.247 ± 0.494) g, respectively ± 0.115), (1.098 ± 0.343) and (1.474 ± 0.644) cm ~ 3, respectively. All P <0.05]. The tumor inhibition rate was significantly different from other treatment groups (P <0.05). In the control group, the cancer cells grew well and the blood vessels were rich. The cancerous tissues in the control group showed different degrees of necrosis and blood vessel components decreased. Immunohistochemical staining showed that the expression of NRP-1 in the control group was significantly higher than that in the treatment groups (P <0.05). The expressions of NRP-1, VEGF and MVD in the combined group were significantly lower than those in other groups (P <0.05). Conclusion: NRP-1 monoclonal antibody combined with docetaxel rhythm chemotherapy may significantly inhibit the growth and angiogenesis of BGC-823 gastric cancer xenografts by down-regulating the expression of NRP-1.