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利用PCR-DGGE技术,研究了裸地及不同连作年限的设施番茄栽培土壤中细菌和真菌的群落结构及多样性的变化情况。结果表明:设施条件下种植番茄明显改变了土壤土著细菌的群落结构,但连作年限对土壤细菌多样性影响较小,细菌群落结构变化不大;土著真菌的群落结构稳定性优于土著细菌,但土壤真菌的优势种群在不同连作年限的土样中变化较大,连作显著降低了某些真菌的数量,同时显著增加了另一些真菌的数量,其中,连作20年番茄的土壤真菌新出现的优势种群最多,且与非优势种群的真菌数量差异较大,这种番茄连作后改变土壤真菌种群平衡的现象可能是导致番茄产生连作障碍的重要原因之一。通过电泳条带的回收、测序,可知所取土样中的细菌优势种群属于Clostridium butyricum、Uncultured bacterium、Bacillus和Arthrobacter,真菌优势种群属于Saccobolus dilutellus、Uncultured fungus、Lasiobolusciliatus、Spooneromyces laeticolor和Lasiobolidium orbiculoides。
Using PCR-DGGE technology, we studied the changes of community structure and diversity of bacteria and fungi in tomato cultivation soil of bare land and different continuous cropping years. The results showed that planting tomato significantly changed the community structure of indigenous bacteria, but the continuous cropping duration had little effect on soil bacterial diversity and the bacterial community structure did not change much. The community structure stability of indigenous fungi was superior to that of indigenous bacteria. The predominant population of soil fungi changed greatly in different successive years of soil samples. The continuous cropping decreased the number of some fungi and significantly increased the number of other fungi. Among them, the newly emerging advantages of soil fungi in tomato continuous cropping for 20 years The population is the largest, and the number of fungi in non-dominant populations is quite different. It may be one of the important reasons for tomato to produce continuous cropping obstacle after changing the balance of soil fungal population after continuous cropping. The dominant species of bacteria were Clostridium butyricum, Uncultured bacterium, Bacillus and Arthrobacter. The dominant fungi belonged to Saccobolus dilutellus, Uncultured fungus, Lasiobolusciliatus, Spooneromyces laeticolor and Lasiobolidium orbiculoides.