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目的:探索舒心胶囊原料药中挥发油的提取工艺。方法:采用高效液相色谱法,用70%甲醇回流提取制样用作阿魏酸含量测定的方法学考察;用水蒸气蒸馏法提取挥发油,以挥发油提取率和阿魏酸含量为指标,采用L(934)正交试验设计,筛选挥发油的最佳提取工艺。结果:舒心胶囊原料药中阿魏酸含量为0.070%。影响提取的主次因素为:浸泡时间>加水量>提取时间;优选得到的最佳提取条件为:药材不必浸泡,加10倍量水(V/W)后提取3h。结论:该工艺准确、稳定,重复性好。
Objective: To explore the extraction process of volatile oil in Shuxin capsule. Methods: High performance liquid chromatography (HPLC) was used to determine the content of ferulic acid by 70% methanol reflux extraction. The volatile oil was extracted by steam distillation. The extraction rate of volatile oil and the content of ferulic acid were used as indexes. (934) orthogonal experimental design, screening the best extraction of volatile oil extraction process. Results: The content of ferulic acid in Shuxin capsule was 0.070%. The main factors affecting the extraction were as follows: soaking time> adding water> extracting time; the optimal extraction conditions were as follows: the medicinal materials did not have to be soaked and extracted for 3h after adding 10 times of water (V / W). Conclusion: The process is accurate, stable and reproducible.