本文利用化学发光法对葡萄糖-葡萄糖氧化酶酶促反应的条件进行了较全面的研究,并利用流动注射化学发光法测定了由酶促反应而产生的H_2O_2的量,从而测定了人体血液中葡萄糖的含量。其方法灵敏度高,特异性好,线性范围宽。对葡萄糖的检测限小于0.1μg/ml,线性范围为0～600mg/dl或更大,相对标准偏差小于4%,对人体血液中葡萄糖的测量结果令人满意。 In this paper, the conditions of enzymatic reaction of glucose-glucose oxidase were studied by using chemiluminescence method. The amount of H 2 O 2 produced by enzymatic reaction was measured by flow injection chemiluminescence. The determination of glucose in human blood Content. The method of high sensitivity, good specificity, linear range. The detection limit of glucose is less than 0.1μg / ml, the linear range of 0 ~ 600mg / dl or more, the relative standard deviation of less than 4%, the human blood glucose was satisfactory.