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目的 通过研究外周免疫刺激后免疫性细胞因子受体在下丘脑神经内分泌核团中的表达变化 ,揭示这些核团与神经免疫调节的关系。 方法 小鼠腹腔内给予细菌内毒素脂多糖 (lipopolysaccharide ,LPS)或葡萄球菌肠毒素B(staphylococcalenterotoxinB ,SEB) ,用免疫组织化学方法观察脾脏核增殖抗体 (PCNA)及下丘脑室旁核和视上核中Ⅰ型IL 1受体的表达 ,并采用双标记技术观察Ⅰ型IL 1受体阳性神经元和加压素及催产素表达的关系。 结果 1 与对照组比较 ,LPS或SEB引起脾脏核增殖抗体的表达明显增强。 2 Ⅰ型IL 1受体在正常小鼠脑内的表达很广泛。与对照组比较 ,LPS或SEB引起Ⅰ型IL 1受体在小鼠下丘脑室旁核和视上核中表达显著增强。 3 在正常下丘脑室旁核和视上核中Ⅰ型IL 1受体阳性的神经元既有加压素能的 ,又有催产素能的。 结论 下丘脑室旁核和视上核参与了免疫反应的调节 ,这两个核团中的部分加压素能神经元和催产素能神经元在神经免疫调节中可能具有重要作用
OBJECTIVE: To investigate the expression of immune cytokines receptors in hypothalamic neuroendocrine nuclei after peripheral immune stimulation, and to reveal the relationship between these nuclei and neuroimmunoregulation. Methods Lipopolysaccharide (LPS) or staphylococcalenterotoxin B (SEB) were intraperitoneally administered to mice. Immunohistochemistry was used to detect the proliferating antibody of the spleen (PCNA) and paraventricular nucleus of the hypothalamus. The expression of IL - 1 receptor in the nucleus and the relationship between IL - 1 receptor type I neurons and the expression of vasopressin and oxytocin were observed by double labeling technique. Results 1 Compared with the control group, LPS or SEB caused splenic nuclear proliferation antibody expression was significantly enhanced. The type 2 IL-1 receptor is widely expressed in the brain of normal mice. Compared with the control group, LPS or SEB induced a significant increase of type I IL-1 receptor expression in the hypothalamic paraventricular nucleus and supraoptic nucleus in mice. 3 In normal hypothalamic paraventricular nucleus and supraoptic nucleus type I IL1 receptor-positive neurons are both vasopressin and oxytocin. Conclusion The hypothalamic paraventricular nucleus and supraoptic nucleus are involved in the regulation of immune response. Some of these two nuclear groups may play important roles in neuroimmunity regulation