绍兴县北海菌种场单正定采用蘑菇单孢分离术和栽培管理法,从“绍丰1号”和“176”蘑菇的子实中分离单孢子获得成功,并用刺激法使分离出的单孢子能够正常萌发。蘑菇一般是以多孢子群散落法繁殖的,但它有病株、弱株多,纯度不高等缺点,采用单孢子分离可以避免这个弱点,但过去由于分离出来的单孢子缺少一种物质刺激,不会萌发,因此无法做种和栽培。1980年底单正定从福建等地学回蘑菇单孢分离术和栽培管理法,从多孢蘑菇菌种中分离单孢子获得 Shaoxing County Beihai bacteria single field positive single mushroom isolation and cultivation management method, from the “Shaofeng 1” and “176” mushroom isolated from the spore success, and the use of stimulus separation The single spore out of the normal germination. Mushrooms usually multiply spore population reproduction method, but it has a diseased strain, weak strains, the purity is not high shortcomings, the use of single spore separation can avoid this weakness, but in the past due to the lack of a single spore isolated substance stimulation, Will not germinate, so can not do planting and cultivation. By the end of 1980 alone from Fujian and other places learn mushroom single spore separation and cultivation management method, isolated from the multi-spore mushroom strain single spore access