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为研究肝素及小肝素分子对培养的平滑肌细胞生长的作用 ,以探讨其是否可以作为一种抗血管平滑肌细胞增殖的药物 ,采用3H -胸腺嘧啶脱氧核苷掺入法观察肝素及小肝素分子对培养的人主动脉平滑肌细胞合成DNA的影响。肝素及小肝素分子的量以糖醛酸表示 ,以其浓度计算 (18、35和 70mg/L)。结果发现 ,肝素及小肝素分子对生长良好的平滑肌细胞有抑制作用 ,不同浓度肝素的抑制率分别为 6 6 %、6 9%和 6 9% ,而不同浓度小肝素分子抑制率分别为 6 0 %、6 8%和 6 7%。肝素及小肝素分子对生长不良的平滑肌细胞有促增殖作用 ,肝素促增殖率为149%、140 %和 180 % ,小肝素分子为 2 0 7%、2 46 %和 30 9%。结果提示肝素及小肝素分子对培养不同状态的平滑肌细胞有抑制或促进增殖的双重调节作用。
In order to investigate the effect of heparin and small heparin molecules on the growth of cultured smooth muscle cells and to explore whether they can be used as a kind of anti - vascular smooth muscle cell proliferation drug, 3H - thymidine incorporation method was used to observe the effect of heparin and small heparin molecules Effects of Cultured Human Aortic Smooth Muscle Cells on DNA Synthesis. The amount of heparin and small heparin molecules is expressed as uronic acid at their concentration (18, 35 and 70 mg / L). The results showed that heparin and small heparin molecules inhibited the growth of good smooth muscle cells. The inhibitory rates of different concentrations of heparin were 66%, 69% and 69%, respectively, while those of different concentrations of heparin were 60% %, 68% and 67%. Heparin and Heparin molecules promote proliferation of poorly-performing smooth muscle cells. Heparin promotes proliferation rates of 149%, 140% and 180%, and small heparin molecules of 207, 246, and 309%. The results suggest that heparin and small heparin molecules can regulate or inhibit the proliferation of smooth muscle cells in different states.