在体外培养的大鼠脑神经细胞上研究了ＮＯ及相关因子对神经细胞的损伤作用。研究表明：（１）ＮＯ的体外神经损伤作用与脂质过氧化作用关系密切；（２）ＮＯ的体外神经损伤作用是由ＮＯ本身的毒性造成的，而与其代谢产物亚硝酸根（ＮＯ２－）和硝酸根（ＮＯ３－）无关；（３）环境酸化可明显促进ＮＯ的体外神经损伤作用。这些结果将十分有助于加深对ＮＯ神经损伤作用的认识 In vitro cultured rat brain neurons were studied NO and related factors on nerve cell injury. The results showed that: (1) the in vitro neurotoxicity of NO was closely related to lipid peroxidation; (2) the neurotoxicity of NO in vitro was caused by the toxicity of NO itself, but not with its metabolite nitrite (NO2-) And nitrate (NO3-) has nothing to do; (3) Environmental acidification can significantly promote NO in vitro nerve injury. These results will be very helpful to deepen the understanding of the role of NO nerve injury