许多药物制剂中,叶酸钙常与其他活性成分配伍,尤其在复合维生素B类产品中,含量较小,定量分析较为困难。作者介绍用弱阴离子交换树脂通过柱层析,以梯度浓度的氯化钠溶液分离及定量测定叶酸钙、维生素B_1、维生素B_6及维生素B_12的混合物,进而用分光光度法测定,有较好的重现性。材料与方法:玻璃层析柱(50cm×2cm内径)有机玻 In many pharmaceutical preparations, folic acid calcium and other active ingredients often compatibility, especially in the complex vitamin B products, the content is small, quantitative analysis more difficult. The authors introduce a weak anion exchange resin column chromatography, gradient sodium chloride solution and quantitative determination of folic acid, vitamin B_1, vitamin B_6 and vitamin B_12 mixture, and then determined by spectrophotometry, and has a better weight Reality Materials and methods: glass column (50cm × 2cm diameter) organic glass