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目的建立瓜果中赤霉素、吲哚乙酸、脱落酸、氯吡脲、2,4-D苯氧乙酸和2甲4氯苯氧乙酸(MCPA)的高效液相色谱法。方法样品经乙酸乙酯超声提取,氮吹至干后,甲醇定容后测定。以甲醇-0.02%磷酸水溶液作为流动相,梯度洗脱,流速1.2ml/min,柱温30℃,经CAPCELL PAK C18色谱柱(250 mm×4.6 mm,5μm)分离,以保留时间和DAD光谱定性,标准曲线法定量。结果赤霉素、2,4-D和MCPA的线性范围均为0.5μg/ml~20μg/ml,吲哚乙酸、脱落酸和氯吡脲的线性范围均为0.1μg/ml~5.0μg/ml,相关系数均大于0.999;6种组分的检出限范围为0.005 mg/kg~0.06 mg/kg;平均回收率范围为82.0%~119%,相对标准偏差(RSD)为0.089%~4.3%。结论该方法操作简便、灵敏、准确,适合于瓜果中6种植物生长调节剂的同时检测。
Objective To establish a high performance liquid chromatography (HPLC) method for the determination of gibberellin, indole acetic acid, abscisic acid, mevalonate, 2,4-D phenoxyacetic acid and MCPA in fruits. Methods The samples were extracted with ethyl acetate by ultrasonic. After the nitrogen was blown to dryness, the volume of methanol was determined. The mobile phase was eluted with methanol-0.02% phosphoric acid as mobile phase with a flow rate of 1.2 ml / min and a column temperature of 30 ℃. The residue was separated on a CAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm) , Standard curve method for quantitative. The linear range of gibberellin, 2,4-D and MCPA was 0.5μg / ml ~ 20μg / ml. The linear ranges of indole acetic acid, abscisic acid and mevalonate were 0.1μg / ml ~ 5.0μg / ml , The correlation coefficients were all greater than 0.999; the detection limits of the six components ranged from 0.005 mg / kg to 0.06 mg / kg; the average recoveries ranged from 82.0% to 119% with relative standard deviations (RSDs) of 0.089% -4.3% . Conclusion The method is simple, sensitive and accurate. It is suitable for the simultaneous detection of six plant growth regulators in fruits and vegetables.