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目的:建立HPLC法测定射干中多种异黄酮类化合物含量的测定方法。方法:采用乙腈-2%磷酸水溶液梯度洗脱,流速:1.0mL/min,进样量:10μL,检测波长:266nm,柱温:25℃,用外标法定量,同时测定中鸢尾苷、野鸢尾苷、鸢尾黄素、野鸢尾黄素、次野鸢尾黄素和白射干素等6种异黄酮类成分的含量。结果:经过HPLC法测定不同产地射干药材的含量以及在根茎与其须根的含量上进行比较,发现彼此之间含量具有很大差异,通过本次实验所得到的各成分含量数值作为参数,能够准确、真实、直观地揭示HPLC法对建立10个不同产地射干药材及须根中多种异黄酮类化合物成分的质量控制的标准性。结论:对不同产地射干中6种异黄酮类成分含量测定结果显示,此法可用于射干药材中6种异黄酮类化合物成分含量的同时测定。
Objective: To establish a method for the determination of multiple isoflavonoids in Shenshu by HPLC. Methods: A gradient of acetonitrile-2% phosphoric acid was used for the gradient elution. The flow rate was 1.0 mL / min. The injection volume was 10 μL. The detection wavelength was 266 nm. The column temperature was 25 ℃. Tectorigenin, iris glycosides, tectorigenin, wild tectorigen, the second wild tectorigenin and white apricot and other six kinds of isoflavones content. Results: The HPLC method was used to determine the contents of Radix scutellariae in different areas and compare the contents of Rhizome and its fibrous roots. The results showed that the contents of Radix scutellariae and Radix were significantly different from each other. By using the content of each component obtained in this experiment as a parameter, Realistically and intuitively, the HPLC method was applied to establish the standard of quality control for a number of isoflavonoid compounds in Chinese medicinal materials and fibrous roots in 10 different areas. Conclusion: The results of determination of 6 isoflavones in Shegan from different areas show that this method can be used for the simultaneous determination of 6 isoflavones in Shegan herb.