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应用染色体分带(C-带)-分子原位杂交技术对普通小麦-中间偃麦草(Thinopyrum intermedium 2n=42,E_1E_1E_2E_2XX)部分双二倍体“中5”(2n=56)的外源染色体进行了鉴定分析。染色体分带结果表明:“中5”的7对中间偃麦草染色体不显带或显示出与受体小麦亲本染色体相似的带型,单靠带型很难准确地鉴定出这7对外源染色体。分带处理后进行分子原位杂交鉴定出“中5”的7对外源染色体,并发现其中一对染色体发生了罗伯逊氏易位(Robertsonian translocation)。表明染色体分带技术与分子原位杂交技术的有机结合,可以大大提高染色体的鉴定水平。
The exogenous chromosomes of 5 “(2n = 56) double diploids of Thinopyrum intermedium 2n = 42, E_1E_1E_2E_2XX were analyzed by chromosome banding (C-band) -hybrid in situ hybridization Accreditation analysis. Chromosomal banding results showed that the 7 pairs of middle-tritrophic chromosomes of ”Zhong 5“ did not show bands or showed similar bands with the recipient wheat parent chromosomes. It was difficult to identify these 7 pairs of exogenous chromosomes accurately by banding. Seven pairs of exogenous chromosomes of ”Zhong 5" were identified by molecular in situ hybridization after banding treatment, and Robertsonian translocation of one pair of chromosomes was found. The results show that the combination of chromosome banding technology and molecular in situ hybridization can greatly enhance the identification of chromosomes.