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[目的]建立石榴皮中熊果酸高效液相色谱测定方法。[方法]采用Diamonsil C18色谱柱(4.6mm×150mm,5μm),甲醇-0.1%乙酸水溶液(pH3.4)(94︰6)为流动相,流速为0.8ml·min-1,检测波长210nm,柱温为室温。[结果]熊果酸在10.4μg·ml-1～104μg·ml-1范围内线性良好,回归方程为Y=16315X+55.119,r=0.9998,平均回收率为99.2%。[结论]该方法简便、准确、重复性好,适用于石榴皮药材中熊果酸的含量测定。
[Objective] To establish a method for the determination of ursolic acid in pomegranate peel by high performance liquid chromatography. [Method] Diamonsil C18 column (4.6mm×150mm, 5μm), methanol-0.1% acetic acid aqueous solution (pH3.4) (94..6) as mobile phase, flow rate of 0.8ml·min-1, detection wavelength at 210nm, The column temperature is room temperature. [Results] The ursolic acid had good linearity in the range of 10.4μg·ml-1 to 104μg·ml-1. The regression equation was Y=16315X+55.119, r=0.9998. The average recovery was 99.2%. [Conclusion] This method is simple, accurate and reproducible, and it is suitable for the determination of ursolic acid in pomegranate peel.