非特异性酯酶(acid α-naphthylacetate esterase,简称ANAE)染色法,在国内外已有不少报道。该法作为人T淋巴细胞标记物,代替E花环试验检查人末稍血T淋巴细胞,已应用于临床。我们在实践中体会到该试验受各种因素的影响,在操作过程中,如稍不适合,就会严重影响结果。为了严格控制试验条件,我们通过反复实践认为应注意以下几点。 Non-specific esterase (acid α-naphthylacetate esterase, referred to as ANAE) staining, at home and abroad have been reported. The law as human T lymphocyte markers, instead of E rosette test of human peripheral blood T lymphocytes, has been used in clinical. We experience in practice that the test by a variety of factors, in the operation process, if not suitable, it will seriously affect the results. In order to strictly control the test conditions, we should pay attention to the following points through repeated practice.