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目的:探讨氧化苦参碱(OM)对SW1990细胞侵袭力的影响.方法:取人胰腺癌细胞SW1990为研究对象,随机分为对照组和OM组,OM组再按照OM剂量不同,分为1,2,4g/L3组.用RT-PCR检测MMP-2mRNA表达变化,以细胞划痕试验和Transwell小室法检测SW1990细胞侵袭力和迁移的变化,用四甲基偶氮唑蓝(MTT)比色法检测OM对SW1990细胞增殖的影响作用.结果:RT-PCR显示MMP-2mRNA在SW1990细胞中的表达,OM各组较对照组显著降低(0.53±0.03,0.42±0.02,0.29±0.03vs0.70±0.03,均P<0.05).细胞侵袭力测定显示,OM各组侵袭过Matrigel胶的细胞数显著低于对照组(325.00±64.43,206.00±84.76,124.00±46.78vs498.00±78.54,均P<0.05),OM组细胞侵袭能力较对照组显著降低.细胞迁移实验结果显示,OM各组穿过滤膜的细胞数显著低于对照组(385.0±58.9,287.0±79.8,186.0±60.9vs586.0±85.8,均P<0.05).结论:OM可能通过下调MMP-2mRNA的表达,抑制胰腺癌细胞侵袭转移能力.
Objective: To investigate the effect of oxymatrine (OM) on invasiveness of SW1990 cells.Methods: The human pancreatic cancer cell line SW1990 was randomly divided into control group and OM group. OM group was divided into 1 group , 2,4 g / L group.The changes of MMP-2 mRNA expression were detected by RT-PCR, the invasiveness and migration of SW1990 cells were detected by cell scratch assay and Transwell chamber assay, respectively. The MTT ratio The colorimetric method was used to detect the effect of OM on the proliferation of SW1990 cells.Results: The expression of MMP-2mRNA in SW1990 cells was significantly decreased by RT-PCR compared with the control group (0.53 ± 0.03,0.42 ± 0.02,0.29 ± 0.03vs0). 70 ± 0.03, all P <0.05) .The cell invasion assay showed that the number of cells in each group of OM invaded Matrigel was significantly lower than that in the control group (325.00 ± 64.43,206.00 ± 84.76,124.00 ± 46.78vs498.00 ± 78.54, both P <0.05), cell invasion ability of OM group was significantly lower than that of the control group.The results of cell migration showed that the number of OM cells in each group was significantly lower than the control group (385.0 ± 58.9,287.0 ± 79.8,186.0 ± 60.9vs586. 0 ± 85.8, all P <0.05) .Conclusion: OM may inhibit the invasion and metastasis of pancreatic cancer cells by down-regulating the expression of MMP-2mRNA.