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目的探讨输注不同浓度聚合人胎盘血红蛋白(Poly PHb)对大鼠造成的氧化损伤。方法建立动物换血模型,将50只SD大鼠随机均分为假手术组:仅做股动/静脉插管处理;氯化钠注射液(对照)组:股动/静脉插管,输注氯化钠注射液行换血实验;2%、4%及6%Poly PHb 3个实验组:股动/静脉插管,分别输注相应浓度的Poly PHb行换血实验。分别在大鼠换血前、换血70%后即刻观测平均动脉压(MAP),换血完成后即刻从大鼠股动脉取血0.5 m L/只,做动脉血血气分析,并检测各组大鼠血浆中还原型谷胱甘肽(GSH)浓度、超氧化物歧化酶(SOD)活力、总抗氧化能力(T-AOC)、丙二醛(MDA)浓度、8-羟基脱氧鸟苷(8-OHDG)浓度。结果 1)大鼠换血70%后,2%、4%及6%Poly PHb组与对照组相比,GSH(μmol/L)分别为33.76±14.33、47.98±21.88、39.73±15.54及10.25±5.70(P<0.01);SOD(U/m L)分别为238.07±27.39、355.73±35.42、405.26±45.01及138.46±57.16(P<0.01);T-AOC(U/m L)分别为20.50±7.87、32.65±4.38、38.03±4.50及5.40±2.22(P<0.01)。2)大鼠换血70%后,2%、4%及6%Poly PHb组与对照组相比,MDA浓度(nmol/m L)随着Poly PHb浓度的增高而增高:45.08±4.12、54.39±3.16、58.52±6.33及2.85±0.59(P<0.01)。3)大鼠换血70%后,2%、4%及6%Poly PHb组与对照组相比,大鼠血浆8-OHDG浓度(nmol/m L)未发生明显变化:2.54!0.90、2.76!0.99、2.79!0.82及2.26!0.42(P>0.05)。结论 Poly PHb对大鼠造成脂质过氧化损伤,且氧化损伤程度随着Poly PHb浓度的增高而加重,但并未对大鼠DNA造成损伤。
Objective To investigate oxidative damage induced by Poly PHb in different concentrations in rats. Methods Fifty Sprague-Dawley rats were randomly divided into sham-operation group: only femoral vein / vein catheterization; sodium chloride injection (control) group: femoral vein / vein catheterization, infusion of chlorine Sodium exchange solution exchange experiments; 2%, 4% and 6% Poly PHb three experimental groups: femoral artery / vein catheterization, respectively, the corresponding infusion of Poly PHb transfusion experiments. The mean arterial pressure (MAP) was observed immediately after the exchange of blood in rats 70% and the blood 0.5 ml / (GSH), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), malondialdehyde (MDA) and 8-OHDG )concentration. Results 1) Compared with the control group, GSH (μmol / L) in 2%, 4% and 6% Poly PHb groups were 33.76 ± 14.33, 47.98 ± 21.88, 39.73 ± 15.54 and 10.25 ± 5.70 (U / m L) were 238.07 ± 27.39, 355.73 ± 35.42, 405.26 ± 45.01 and 138.46 ± 57.16 respectively (P <0.01), and the T-AOC values were 20.50 ± 7.87 , 32.65 ± 4.38, 38.03 ± 4.50 and 5.40 ± 2.22 (P <0.01), respectively. 2) The concentration of MDA (nmol / m L) in 2%, 4%, and 6% Poly PHb group increased with the increase of Poly PHb concentration when rats transfused 70%: 45.08 ± 4.12,54.39 ± 3.16, 58.52 ± 6.33 and 2.85 ± 0.59 (P <0.01). 3) In rats of 2%, 4%, and 6% Poly PHb group, there was no significant change of 8-OHDG concentration (nmol / m L) in rats after 70% exchange of blood: 2.54! 0.90,2.76! 0.99, 2.79! 0.82 and 2.26! 0.42 (P> 0.05). Conclusion Poly PHb can cause lipid peroxidation injury in rats, and the degree of oxidative damage aggravates with the increase of Poly PHb concentration, but it does not cause damage to rat DNA.