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本研究采用兔离子减薄工艺,制备出人正常牙釉质表层区的电子透射超薄区,在 JEOL TEM-200CX 高分辨率透射电镜下观察。结果显示,釉质表面呈现起伏波纹,周期约为175nm,有的视野见个别微晶突出于表面。表层微晶宽约24nm,长轴互相平行,垂直于表面;微晶之间存在明显的晶粒间微隙,宽1～30nm,平均8nm,有的直接向釉质表面开放。并观察到许多散在晶粒,其形态、大小及分布提示为再矿化晶粒。另外在表层部分区域见不规整现象,主要表现为晶粒短小,排列紊乱,晶粒间空隙增大,甚至成为空洞,认为属表层内的结构缺陷。
In this study, rabbit ion thinning process was used to prepare ultra-thin electron-transmissive region of human normal enamel surface area and observed under high-resolution transmission electron microscope (TEM) with JEOL TEM-200CX. The results show that the enamel surface presents undulating ripples, a period of about 175nm, some fields of view of individual crystallites protruding from the surface. The surface crystallites are about 24nm in width with their long axes parallel to each other and perpendicular to the surface. There are obvious intergranular micro-gaps between the crystallites, which are 1 ~ 30nm wide with an average of 8nm. Some of them are directly open to the enamel surface. Many interspersed grains were observed and their morphology, size and distribution suggested remineralization grains. In addition, see some irregular phenomenon in the surface area, mainly manifested as short grains, disordered arrangement, the gap between the grains increases, or even become hollow, that is, structural defects within the surface layer.