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为了将虎杖作为饲料添加剂应用于水产养殖中,以血鹦鹉(Cichlasoma citrinellum♂×C.synspilum♀)(体质量为48.0 g±3.5 g)为试验对象,以鱼粉、豆粕等为主要蛋白源,鱼油为主要脂肪源,配制脂肪水平分别为8%、14%的基础饲料和高脂饲料,并在高脂饲料中添加不同含量的虎杖(质量分数为0、0.5%、1.0%、1.5%、2.0%),共分6个处理组进行投喂,每个处理组设3个平行,试验周期为28 d,分别于试验开始后第7、14、21、28天取样测定血鹦鹉部分非特异性免疫与脂类代谢指标。结果表明:与对照组相比,随着虎杖添加量的增加,血鹦鹉肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶(LZM)活力显著提高(P<0.05),丙二醛(MDA)含量呈降低趋势,血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)活力随虎杖添加量的增加呈降低趋势,试验结束时,在最大添加量2.0%时与对照组有显著性差异(P<0.05),血清中甘油三酯(TG)、总胆固醇(CHOL)含量随虎杖添加量的增加呈降低趋势,但与对照组无显著性差异(P>0.05),而血糖(Glu)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)含量变化不明显(P>0.05);肝脏中脂蛋白脂酶(LPL)、肝脂酶(HL)活力随虎杖添加量的增加呈下降趋势,但无显著性差异(P>0.05),脂肪酸合成酶(FAS)活力随虎杖添加量的增加而显著降低(P<0.05)。研究表明,饲料中添加虎杖可提高血鹦鹉肝脏抗氧化能力与溶菌酶活力,减轻肝损伤,还可增强血鹦鹉的脂类代谢能力,建议血鹦鹉饲料中虎杖的添加量为2.0%,连续喂食14 d时效果最佳。
In order to use Polygonum cuspidatum as a feed additive in aquaculture, fish (Cichlasoma citrinellum♂ × C.synspilum♀) (body weight 48.0 g ± 3.5 g) was used as the test object. Fish meal and soybean meal were used as main protein sources, As the main fat source, the basic fat and high fat diet with the fat level of 8% and 14% were respectively prepared, and different contents of Polygonum cuspidatum were added into the high fat diet (mass fraction 0,0.5%, 1.0%, 1.5%, 2.0 %) Were divided into 6 treatment groups for feeding. Each treatment group was set to 3 parallel with a test period of 28 days. Samples of non-specific immunity of blood parrot were sampled at the 7th, 14th, 21st and 28th days after the start of the test And lipid metabolism indicators. The results showed that compared with the control group, the activities of superoxide dismutase (SOD), catalase (CAT) and lysozyme (LZM) in liver increased significantly with the increase of Polygonum cuspidatum (P <0.05) , Malondialdehyde (MDA) content decreased, serum ALT and AST activities tended to decrease with the addition of Polygonum cuspidatum. At the end of the experiment, when the maximum dosage was 2.0% (P <0.05). The content of triglyceride (TG) and total cholesterol (CHOL) in serum decreased with the increase of Polygonum cuspidatum, but there was no significant difference with the control group (P> 0.05) The content of Glu, HDL and LDL did not change significantly (P> 0.05). The activities of LPL and HL in the liver were increased with the addition of Polygonum cuspidatum (P> 0.05). The activity of fatty acid synthase (FAS) was significantly decreased with the addition of Polygonum cuspidatum (P <0.05). Studies have shown that the addition of Polygonum cuspidatum feed can improve the anti-oxidative capacity of liver and anti-lysozyme activity of blood parrot to reduce liver damage, but also enhance the lipid metabolism of blood parrots, suggesting that the blood Parrot feeding Polygonum cuspidatum 2.0%, continuous feeding Best effect on 14 d.