论文部分内容阅读
目的 探讨刺五加叶皂甙（ Ａ Ｓ Ｓ）诱导肝癌细胞凋亡和抑制宿主肝癌生长和转移的作用，以寻找治疗肝癌的新途径。方法 给 Ｂ Ａ Ｂ Ｌ／ Ｃ 小鼠腹腔内注射 Ｈ ｅｐ Ｇ ２ 肝癌细胞，１０ ｄ 后给予 Ａ Ｓ Ｓ 腹腔注射，８ ｗ 后处死实验动物，取瘤称重，通过抑瘤率判断 Ａ Ｓ Ｓ 小鼠移植癌的抑制作用，流式细胞仪检测移植肝癌组织凋亡小体百分率。结果 当 Ａ Ｓ Ｓ 剂量为 ０２５ ｍ ｇ／ｋｇ 和 １ ｍ ｇ／ｋｇ时，抑瘤率分别为 ４５４３％ 和 ７２０６％ ，凋亡小体百分率为 ２０６±３９％ ，３１１±７８％ 。 Ａ Ｓ Ｓ 组腹膜、肝、肠系膜和脾的癌转移率显著低于对照组。结论 Ａ Ｓ Ｓ 有抑制人肝癌在小鼠体内生长和转移的作用，其作用随剂量增加而递增，此作用可能与 Ａ Ｓ Ｓ 促进肝癌细胞凋亡有关。
Objective To investigate the effect of Acanthopanax senticosus saponins (A S S) on apoptosis of hepatocellular carcinoma cells and inhibition of the growth and metastasis of host hepatoma, in order to find new ways to treat liver cancer. Methods B A B L / C mice were injected intraperitoneally with H ep G 2 hepatocarcinoma cells. After 10 days, A S S was given intraperitoneally. After 8 weeks, the experimental animals were sacrificed. The tumors were weighed, and the A S S was judged by the inhibition rate. Inhibitory effect of transplanted cancer in mice, flow cytometry was used to detect the percentage of apoptotic bodies in transplanted hepatocellular carcinoma. Results When A S S doses were 025 mg/kg and 1 mg/kg, the tumor inhibition rates were 4543% and 7206%, respectively. The percentage of apoptotic bodies was 206±39. %, 311±78%. The metastatic rate of peritoneum, liver, mesentery, and spleen in the A S S group was significantly lower than that in the control group. Conclusion A S S can inhibit the growth and metastasis of human hepatoma in mice. The effect of A S S increases with increasing doses, which may be related to A S S-induced apoptosis of hepatoma cells.