论文部分内容阅读
目的探讨锰能否诱导SH-SY5Y细胞发生线粒体自噬。方法以人神经母细胞瘤SH-SY5Y细胞为模型,不同剂量的Mn Cl2处理细胞24 h,通过MTT法检测Mn Cl2对细胞存活率的影响,流式细胞仪检测Mn Cl2对细胞线粒体膜电位的影响,以Mito-Tracker Green和Lyso-Tracker Red荧光探针分别标记线粒体和溶酶体,激光共聚焦显微镜观察细胞线粒体和溶酶体的共定位,以表征线粒体自噬。结果 Mn Cl2可剂量依赖性地降低SH-SY5Y细胞存活率,并降低细胞线粒体膜电位,Mn Cl2诱导细胞线粒体和溶酶体共定位。结论 Mn Cl2可诱导SH-SY5Y细胞发生线粒体自噬,线粒体自噬可能参与锰神经毒性机制。
Objective To investigate whether manganese induces mitochondrial autophagy in SH-SY5Y cells. Methods Human neuroblastoma SH-SY5Y cells were used as a model and treated with different doses of MnCl 2 for 24 h. The effect of MnCl 2 on cell viability was examined by MTT assay. The effect of MnCl 2 on mitochondrial membrane potential Mitochondria and lysosomes were labeled with Mito-Tracker Green and Lyso-Tracker Red fluorescent probes respectively. The co-localization of mitochondria and lysosomes was observed by laser confocal microscopy to characterize mitochondrial autophagy. Results Mn Cl2 could reduce the survival rate of SH-SY5Y cells in a dose-dependent manner and reduce the mitochondrial membrane potential. MnCl 2 induced mitochondrial and lysosome colocalization. Conclusion Mn Cl2 can induce mitochondrial autophagy in SH-SY5Y cells. Mitochondrial autophagy may participate in the neurotoxicity of manganese.