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目的观察链脲佐菌素(STZ)与四氧嘧啶(ALX)诱导ICR小鼠高血糖模型在降血糖实验中的效果差异,探讨建模和血糖测定的最佳方法。方法选择ICR小鼠90只,随机分为2组,分别用STZ和ALX生理盐水溶液腹腔注射造模,6天后依血糖值分为4组,即STZ干预组、STZ模型组和ALX干预组、ALX模型组。用一保健食品对干预组经口给药30天,模型组给予纯净水,进行糖耐量实验,观察每组的血糖值变化。结果两种药物在造模前后的体重、血糖浓度、成模率和死亡率均无显著性差异(P>0.05);但ALX组造模6天血糖水平高于STZ组,有生物学意义。糖耐量实验中血糖仪出现HI值(异常高值)数量表明,0.5h时,ALX模型组明显少于STZ模型组(P<0.01);ALX干预组出现HI值数量少于STZ干预组((P<0.05);综合两者出现率,ALX组明显少于STZ组(P<0.01)。在0h和2h两个时点,ALX组也有少于STZ组的现象并有趋向显著性之势。结论 ALX造模速度快,动物的高血糖浓度较稳定且持续时间较长,仍然是建立高血糖动物模型的首选药物。
Objective To observe the effect of streptozotocin (STZ) and alloxan (ALX) on the hypoglycemic effect of ICR mouse model of hyperglycemia, and to explore the best method of modeling and blood glucose determination. Methods Ninety ICR mice were randomly divided into 2 groups. They were injected intraperitoneally with STZ and ALX saline solution respectively. After 6 days, they were divided into 4 groups according to the blood glucose level: STZ intervention group, STZ model group and ALX intervention group. ALX model group. With a health food to the intervention group for 30 days by oral administration, the model group was given purified water, glucose tolerance test, observe the changes in blood glucose values of each group. Results There were no significant differences in body weight, blood glucose concentration, morbidity and mortality between the two drugs before and after modeling (P> 0.05). However, the blood glucose levels of ALX group at 6 days were higher than that of STZ group, which had biological significance. The number of HI values (abnormally high values) of glucose meters in the glucose tolerance test showed that the ALX model group was significantly less than the STZ model group (P <0.01) at 0.5h; the HI value in the ALX intervention group was less than that of the STZ intervention group P <0.05), and the incidence of both groups was significantly lower in ALX group than in STZ group (P <0.01) .At the time of 0h and 2h, ALX group also had less tendency than STZ group and had a trend of significant tendency. Conclusion ALX modeling speed, animals with high blood sugar concentration more stable and longer duration, is still the establishment of high blood sugar animal model of choice drug.