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目的测定坚杆火绒草及其不同炮制品中绿原酸、咖啡酸和木犀草素的含量。方法采用Acquity UPLC BEH C18色谱柱(50 mm×2.1 mm,1.7μm),流动相A为甲醇、B为0.1%磷酸水溶液,梯度洗脱,柱温35℃,流速0.2 m L·min~(-1),进样量1μL,多波长分段检测。结果绿原酸、咖啡酸和木犀草素的线性范围分别为:3.68~73.60 ng(r=0.9999)、1.00~20.00 ng(r=0.9999)、0.90~40.50 ng(r=0.9999)。坚杆火绒草中绿原酸、咖啡酸和木犀草素的含量差异较大,同一植株的叶、花和果实中绿原酸和咖啡酸的含量明显高于根和茎中的;根和茎中均未检测到木犀草素,而花和果实中均含有木犀草素。结论藏医火灸柱水煮后晒干的工艺不建议继续使用。
Aim To determine the content of chlorogenic acid, caffeic acid and luteolin in the processed products of Ardisia tenuiflora and its different processed products. Methods The mobile phase A was methanol and the gradient elution was performed on a Acquity UPLC BEH C18 column (50 mm × 2.1 mm, 1.7 μm) with a mobile phase A of 0.1% phosphoric acid. The column temperature was 35 ℃ and the flow rate was 0.2 mL · min ~ 1), injection volume 1μL, multi-wavelength segment detection. Results The linear ranges of chlorogenic acid, caffeic acid and luteolin were 3.68-73.60 ng (r = 0.9999), 1.00-20.00 ng (r = 0.9999) and 0.90-40.50 ng (r = 0.9999), respectively. The contents of chlorogenic acid, caffeic acid and luteolin differed greatly in F. tenuiflora. The contents of chlorogenic acid and caffeic acid in leaves, flowers and fruits of the same plant were significantly higher than those in roots and stems. Were not detected in the luteolin, flowers and fruits contain luteolin. Conclusion Tibetan medicine fire moxibustion column after the drying process is not recommended to continue to use.