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一、概述1964年 Jamieson 和 Palade 发现哺乳动物(包括人)的心房肌细胞中含有一种特殊颗粒,认为心房肌细胞除有收缩功能外,可能还有形成、贮存、分泌这种颗粒的功能。1978年 De Bold 发现这些心房颗粒可能是含色氨酸和含硫氨基酸的蛋白质或肽类。1981年 De Bold 将心房提取物注射于大鼠引起迅速强大的利钠、利尿作用。证明心房含有一种心房利钠肽(Atrial natriuretic peptide,ANP)或称心房利钠因子(Atrial natriureticfactor,ANF)。直到1984年,人和大鼠的 ANP才被分离、提纯并确定了其氨基酸排列序列。同年又被人工合成并制备了 ANP 抗体,建立了 ANP 放射免疫测定(RIA)方法,从而把 ANP 的研究推向了新阶级。现已知人的 ANP 有α、β、γ三种,分别由28、56、126个氨基酸组成。低分子的α-
I. Overview In 1964, Jamieson and Palade found that mammalian (including human) atrial myocytes contain a special type of granules that, in addition to contractile function, may function to form, store and secrete such granules. In 1978, De Bold found that these atrial particles may be proteins or peptides containing tryptophan and sulfur-containing amino acids. In 1981, De Bold injected atrial extract into rats causing rapid and powerful sodium and diuretic effects. Atrial natriuretic peptide (ANP) or atrial natriuretic factor (ANF) is demonstrated in the atria. Until 1984, human and rat ANP was isolated, purified and identified its amino acid sequence. In the same year, it was artificially synthesized and prepared ANP antibody, established ANP radioimmunoassay (RIA) method, which ANP research to a new class. Now known as ANP α, β, γ three, respectively, by 28,56,126 amino acids. Low molecular weight α-