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目的 :风湿康胶囊中挥发油质量的稳定性关系到整个制剂的疗效 ,因此必须对之进行质量控制。方法 :采用水提法制备样品 ,先用水使挥发油从β -CD包合物中释放出 ,然后用石油醚萃取。气相色谱条件为 :DB - 1毛细管 2 5m× 0 32mmID× 0 5 2 μm ,载气N2 流速 2 5mL·min- 1 ,空气流速 2 5 0mL·min- 1 ,氢气流速 2 5mL·min- 1 ,尾吹气 2 0mL·min- 1 ,分流比 70∶1,进样量 6 μL ,进样温度 2 2 0℃ ,检测温度 32 0℃ ,柱温 15 0℃维持 14min ,以 30℃·min- 1 升温至 2 80℃ ,并维持 12min。结果 :以藁本内酯为研究代表 ,该制剂中的挥发油在 3个月内是稳定的。结论 :毛细管气相色谱法测定藁本内酯含量的方法简单可行 ,所需样品量少。
OBJECTIVE: The stability of the volatile oil in Fengruikang capsule is related to the curative effect of the whole preparation, so it must be controlled by quality. Methods: Samples were prepared by water extraction. The volatile oil was first released from the β-CD inclusion complex with water and then extracted with petroleum ether. The gas chromatographic conditions were as follows: DB - 1 capillary tube 2 5m × 0 32mmID × 0 5 2 μm, carrier gas N2 flow rate 25mL · min -1, air flow rate 250mL · min -1, hydrogen flow rate 2.5mL · min -1, The injection temperature was 220 ℃, the detection temperature was 32 ℃, the column temperature was maintained at 150 ℃ for 14min, and the flow rate was 30 ℃ · min- 1 Warmed to 280 ℃, and maintained 12min. Results: With ligustilide as a representative study, the volatile oil in this preparation is stable within 3 months. Conclusion: Capillary gas chromatography method for the determination of ligustilide content is simple and feasible method, the required sample size.