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对1985～1989年广州市临床诊断为登革热(DF)病人采集血清和捕捉蚊媒,应用C6/36白纹伊蚊细胞系分离病毒和血清学检测。结果表明,1985年分离得5株1型DF病毒。1986～87年分离得39株2型DF病毒。1985年从白纹伊蚊分离获得1株1型DF病毒,首次证实白纹伊蚊是广州市DF流行的主要传播媒介。1986年从致乏库蚊分离得4株非DF病毒,经电镜等检测初步鉴定为披膜病毒。1988～1989年,从病原学,血清学监测,广州市未发生DF流行。通过5年系统地监测,初步了解到广州DF流行传染源属外来性,通过本地媒介而发生流行的特点。
Serum and mosquito media were collected from Dengue fever (DF) patients in Guangzhou during 1985 ~ 1989 and virus and serological tests were performed using C6 / 36 Aedes albopictus cell lines. The results showed that in 1985, five strains of type 1 DF were isolated. From 1986 to 1987, 39 strains of type 2 DF viruses were isolated. In 1985, one type 1 DF virus was isolated from Aedes albopictus, and it was first confirmed that Aedes albopictus was the main transmission medium of DF epidemic in Guangzhou. In 1986, 4 non-DF viruses were isolated from Culex pipiens, and preliminary detection of togavirus by electron microscopy. From 1988 to 1989, from the etiology, serological surveillance, Guangzhou did not occur DF epidemic. After 5 years of systematic monitoring, it is initially understood that the source of epidemic spread of DF in Guangzhou originates from the externalities and prevails through the local media.