脂质A是革兰阴性菌外膜的一个关键组成部分,通过Toll样受体4(TLR4)-MD2-CD14途径刺激促炎反应。它的内毒素活性依赖于酰基链的数量和长度以及其磷酸化的状态。在鼠伤寒沙门菌中,除去脂质A中的次级月桂酸酯或肉豆蔻酸酯链导致细菌的减毒和在体外的生长缺陷。然而,脂质A的这两个磷酸基团,在细菌毒力和免疫原性中所起的作用仍然未知。在这里,我们用携带有五酰基化脂 Lipid A is a key component of the outer membrane of Gram-negative bacteria and stimulates proinflammatory responses via the Toll-like Receptor 4 (TLR4) -MD2-CD14 pathway. Its endotoxin activity depends on the number and length of acyl chains and their phosphorylation status. In S. typhimurium, removal of the secondary laurate or myristate chain in lipid A resulted in attenuation of bacteria and growth defects in vitro. However, the role of these two phosphate groups of lipid A in bacterial virulence and immunogenicity remains unknown. Here, we carry a five acylated lipid